Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry

Weijia William Wu; Michael J McPhaul; Zengru Wu

doi:10.1007/978-1-0716-2565-1_43

Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry

Methods Mol Biol. 2022:2546:485-492. doi: 10.1007/978-1-0716-2565-1_43.

Authors

Weijia William Wu¹, Michael J McPhaul¹, Zengru Wu²

Affiliations

¹ Quest Diagnostics, San Juan Capistrano, CA, USA.
² Quest Diagnostics, San Juan Capistrano, CA, USA. zeng.r.wu@questdiagnostics.com.

PMID: 36127615
DOI: 10.1007/978-1-0716-2565-1_43

Abstract

A method for free thyroxine measurement in human serum using equilibrium dialysis followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) is described. Free thyroxine in serum is first separated from protein-bound thyroxine by equilibrium dialysis and then measured by LC-MS/MS.

Keywords: Equilibrium dialysis; Free thyroxine; LC-MS/MS; T4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid / methods
Humans
Renal Dialysis
Tandem Mass Spectrometry* / methods
Thyroxine*

Substances

Thyroxine